Journal: Renal Failure

Article Title: Efficacy of nicotiflorin in ameliorating septic acute kidney injury: the role of PINK1/parkin in mitochondrial restoration and oxidative stress reduction

doi: 10.1080/0886022X.2026.2624206

Figure Lengend Snippet: The effect of nicotiflorin on mitophagy in vivo. Mice were treated with/without LPS (10 mg/kg) and/or nicotiflorin (50 mg/kg) for 24 h. (A) After sacrifice of mice, kidney tissues were excised and underwent IF staining to detect PINK1 and LC3 signals (×400 magnification, scale bar: 100 µm). (B-F) Protein levels of PINK1, Parkin, LC3II/LC3I and p62 in kidney tissues were measured by Western blot. β-actin served as the loading control. *** p < 0.001 vs. Control; ^ p < 0.05, ^^ p < 0.01 vs. Nicotiflorin; # p < 0.05, ### p < 0.001 vs. LPS. n = 3, measurement data expressed as mean ± standard deviation. Difference comparisons among multiple groups were analyzed using one-way analysis of variance. PINK1: PTEN-induced putative protein kinase 1; LPS: lipopolysaccharide.

Article Snippet: Mitophagy Detection Kit (MD01, Dojindo, Tokyo, Japan) was used for detecting changes in mitophagy in NRK-52E cells as previously described [ ].

Techniques: In Vivo, Staining, Western Blot, Control, Standard Deviation

Journal: Renal Failure

Article Title: Efficacy of nicotiflorin in ameliorating septic acute kidney injury: the role of PINK1/parkin in mitochondrial restoration and oxidative stress reduction

doi: 10.1080/0886022X.2026.2624206

Figure Lengend Snippet: The effect of nicotiflorin on mitophagy in LPS-treated NRK-52E cells. NRK-52E were treated with/without LPS (10 µg/ml) and/or nicotiflorin (75 µg/ml) for 24 h. (A–B) The cells were treated with JC-10 fluorescent probe and analyzed by flow cytometer to measure mitochondrial membrane potential. (C) The mitophagy in the cells was determined by Mitophagy Dye combined with confocal microscope (×200 magnification, scale bar: 100 µm). (D–E) The degree of mitochondria fused to lysosomes was analyzed by flow cytometry after the cells were treated with Mitophagy Dye and Lyso Dye. * p < 0.05, *** p < 0.001 vs. Control; ^^ p < 0.01, ^^^ p < 0.001 vs. Nicotiflorin; # p < 0.05, ### p < 0.001 vs. LPS. n = 3, measurement data expressed as mean ± standard deviation. Difference comparisons among multiple groups were analyzed using one-way analysis of variance. LPS: lipopolysaccharide.

Article Snippet: Mitophagy Detection Kit (MD01, Dojindo, Tokyo, Japan) was used for detecting changes in mitophagy in NRK-52E cells as previously described [ ].

Techniques: Flow Cytometry, Membrane, Microscopy, Control, Standard Deviation

Journal: Renal Failure

Article Title: Efficacy of nicotiflorin in ameliorating septic acute kidney injury: the role of PINK1/parkin in mitochondrial restoration and oxidative stress reduction

doi: 10.1080/0886022X.2026.2624206

Figure Lengend Snippet: The regulatory mechanism of nicotiflorin in apoptosis, oxidative stress and mitophagy in LPS-treated NRK-52E cells. NRK-52E cells were treated with/without LPS (10 µg/ml) and/or nicotiflorin (75 µg/ml) for 24 h. (A–L) Western blot was performed to measure protein levels of Bcl-2, Bax, Cleaved caspase-3, Cyt C, Nrf2, KEAP1, PINK1, Parkin, LC3II/LC3I and p62 in the cells. β-actin served as the loading control. * p < 0.05, *** p < 0.001 vs. Control; ^^ p < 0.01, ^^^ p < 0.001 vs. Nicotiflorin; ## p < 0.01, ### p < 0.001 vs. LPS. n = 3, measurement data expressed as mean ± standard deviation. Difference comparisons among multiple groups were analyzed using one-way analysis of variance. Bcl-2: B-cell lymphoma-2; Bax: BCL2 Associated X; Cyt C: Cytochrome C; Nrf2: NF-E2-related factor-2; KEAP1: kelch-like ECH associated protein 1; PINK1: PTEN-induced putative protein kinase 1; LPS: lipopolysaccharide.

Article Snippet: Mitophagy Detection Kit (MD01, Dojindo, Tokyo, Japan) was used for detecting changes in mitophagy in NRK-52E cells as previously described [ ].

Techniques: Western Blot, Control, Standard Deviation